One way to look for auxotrophic mutants in microbes is to inoculate thousands of mutagenized…

One way to look for auxotrophic mutants in microbes is to inoculate thousands of mutagenized colonies in specific positions on plates of fully supplemented medium, then to use a pad of sterile felt to transfer cells from these colonies into similar positions on plates of minimal medium (“replicaplating”). Auxotrophs do not grow on minimal medium. Would you call this a selection or a screen for auxotrophs? Explain.

 

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